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AbstractFR.02.07 Psoriasin at the ocular surface Garreis F.1, Gottschalt M.1, Gläser R.2, Worlitzsch D.3, Paulsen F.1
1Department of Anatomy and Cell Biology and 3Department of Hygiene, Martin-Luther-University, Halle-Wittenberg, Halle (Saale); 2Department of Dermatology, Christian-Albrechts-University of Kiel, Kiel The 11-kDa metal ion-binding (Zn2+) S100 peptide psoriasin (S100A7), has been identified as a principal E-coli-killing antimicrobial peptide of healthy human skin. The aim of our study was to investigate the expression und the function of psoriasin at the ocular surface. Different tissues of the lacrimal apparatus (Meibomian gland, nasolacrimal ducts) and ocular surface (cornea and conjunctiva) were analysed by means of RT-PCR, Western blot and immunohistochemistry for their ability to express and produce psoriasin. Moreover, inducebility of psoriasin was analysed in three epithelial cell lines, a sebocyte (SCL), a corneal (HCE) and a conjunctival epithelial cell line (HCjE) after challenge with frequent ocular pathogens as well as different concentrations of interleucin (IL)-1b, vascular endothelial growth factor (VEGF) and trefoil factor family peptide 3 (TFF3). Real-time PCR and ELISA experiments were performed to evaluate the effect on the inducibility of psoriasin.
Additionally, we examined tear fluid obtained from different healthy persons for its psoriasin concentration by ELISA. RT-PCR and Western blot revealed expression and presence of psoriasin in cornea, conjunctiva and nasolacrimal ducts but not in lacrimal gland. Psoriasin could be immunolocalized in the epithelium of conjunctiva, around hair follicles as well as in of Meibomian glands but was absent in lacrimal gland. No induction of psoriasin was observed after stimulation with supernatants of different bacteria and TFF3 whereas IL-1b and VEGF strongly induced psoriasin. Highest amounts of psoriasin were detected in lacrimal fluid (~170 ng/ml) and Meibomian glands. These results suggest that psoriasin may play a protective role at the ocular surface and is part of the innate immune system of the ocular surface. Moreover, an involvement in angio- or antiangiogenesis maybe hypothezised.
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