Abstract

SO.19.09

Loss of Ptf1a gene leads to transdifferentiation of amacrine precursor cells to ganglion cells

Sel S.¹, Nakhai H.², Kaliniski T.³, Ehrich D.⁴, Enssen I.¹, Sasse A.¹, Vetter E.¹, Bräuer L.⁵, Duncker G. I. W.¹, Paulsen F.^5
1Klinik und Poliklinik für Augenheilkunde, ⁵Institut für Anatomie und Zellbiologie, Universität Halle/Wittenberg; ²II. Medizinische Klinik und Poliklinik, Klinikum rechts der Isar der Technischen Universität München; ³Institut für Pathologie der Universität Magdeburg; ⁴Augenklinik Vogtland-Klinikum Plauen 

Objective: Ptf1a plays a pivotal role for the retinogenesis. In this study we analyse further functions of Ptf1a during the development of the retina.
Methods: Using the Cre-loxP-recombination system we have generated a Ptf1a^Cre/+ mice line and crossed to Z/EG reporter mice line (express GFP-Protein upon excision of a stop codon by Cre) in order to trace the Ptf1a-expressing cells during the development of the mouse retina.
Results: We show that the conditional knock-out of Ptf1a leads to transdifferentiation of amacrine precursor cells to ganglion cells. Moreover, we found that Ptf1a is involved in the signalling cascade of cell-cell contacts.
Conclusions: These results show that Ptf1a is crucial for the development of amacrine cells during retinogenesis.

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