Abstract

P 081

Inhibition of human lens epithelial cells (hLEC) in vitro in an organ culture model using a pharmacologically modified haptic of artificial lenses

Kwittner S.¹, Weidmann A.², Nebe B.²,Teller J.³, Beck R.^1
1Department of Ophthalmology, ²Department of Internal Medicine, Clinical Research/Cell Biology, University of Rostock, Rostock, ³Micromod GmbH, Rostock

Objective: The prevention of the cataracta secundaria is still a major problem of the ophthalmology. Therfeore, the aim of our study was to investigate in vitro the efficacy of a pharmacologically modified haptic of artificial lenses using mibefrail in an aftercataract organ culture model.
Methods: The calcium channel blocker mibefradil dihydrochloride (Sigma) was encapsulated in spheric poly-lactic-co-glycolic-acid (PLGA) micro particles by Micromod^® (Rostock) for application in vitro. These micro particles were chemically coupled to the haptic. HLEC were cultured in postmortem lens capsules. After implantation of the pharmacologically modified haptic the influence of the encapsulated substance on lens cells was studied by measuring metabolism (MTT) and cell survival (live/dead-viability test) as well as microscopically
Results: The metabolism of lens epithelial cells showed a decrease after 24h with the immobilized mibefradil. The live/dead-viability test underlines these effects. The morphology of cells was impaired and the cell area reduced.
Conclusions: It is feasible to encapsulate the calcium channel blocker mibefradil, to immobilize the capsules at the haptic of artificial lenses and to get a release behavior effecting cell viability and growth. This approach using mibefadil containing micro particles with a continuously released agent seems to be a practicable approach to prevent cataracta secundaria.

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