Abstract

P 073

SELDI ProteinChip analysis of tearprotein changes in tears of patients with allergic conjunctivitis with and without therapy of antihistamines in comparison with healthy patients

Lang M.¹, Wiegel N.², Grus F. H.^2 
1Eye clinic of Darmstadt, ²Johannes-Gutenberg-University, Department of Ophthalmology, Mainz

Objective: The diagnosis of allergic conjunctivitis is usually based on the clinical symptoms, the IgE-Lacrytest and cytological test. There are also systemic tests, but for the diagnosis of the allergic conjunctivitis, they are often inexact.
With the SELDI ProteinChip technology we tried to find out, if there are any specific tear protein changes in allergic conjunctivis, to be used as a biomarker.
Methods: 128 eyes were examined (74 with allergic conjunctivitis without therapy and 54 with antihistamine therapy). Tears were examined by SELDI ProteinChip technology and the results were compared with tears of healthy patients.
Results: The computer analysis showed a significant difference in the tears between allergic patients and healthy patients. Most of the tear proteins showed a lowering of the concentration.
Conclusions: It was possible to show, that this was an easier approach to the diagnosis of allergic conjunctivitis. In comparison to SDS- electrophoresis there was a higher discrimination of tear proteins especially in lower molecular proteins.

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