Abstract

DO.08.05

Langerhans cells in clinical diagnostic – confocal microscopy and immunohistochemistry

Zhivov A., Guthoff R. F. 
University Eye Clinic Rostock

Langerhans cells (LC) are a critical factor in antigen presenting function in cornea and conjunctiva. LC might be an important regulator of immune answer and modulate between immune defence und immune tolerance. Corneal Langerhans cells are an issue of ongoing experimental studies. Until now was it impossible to investigate these cells in vivo. With the help of in vivo confocal microscopy was possible to show, that corneal LC are present both central and peripheral. Moreover, LC present as either large cells bearing long processes or smaller cells lacking cell dendrites, most supposedly indicating mature and immature phenotype, respectively. The identification and distribution of the LCs was performed immunohistochemically. Quantitative evidence of LC in cornea and conjunctiva could be of clinical relevance in evaluation of wound healing, graft versus host disease, allergic and toxic drug side effects, etc. The aim of the lecture is to present the modern diagnostic possibilities of in vivo confocal microscopy und immunohistochemistry regarding LC in healthy volunteers, contact lens wearers, glaucoma, dry eye patients, etc.

Copyright: Porstmann Kongresse GmbH. All rights reserved. Impressum, Disclaimer.