Abstract

SA.08.02a

Toxicity of light induced Indocyanine green decomposition products

Hillenkamp J.¹, Kobuch K.¹, Maisch T.¹, Baumler W.², Vasold R.³, Szeimies R. M.², Helbig H.¹
Departments of ¹Ophthalmology, ²Dermatology, and ³Organic Chemistry, Regensburg University

Objective: To investigate cytotoxicity of Indocyanine green (ICG) decomposition products.
Methods: ICG was dissolved in Glucose 5% at 0.01%, 0.025% and 0.05% concentrations. Decomposition of ICG was induced by illumination with a diode laser (805 nm). The decomposition and its products were identified by HPLC and mass spectrometry. Cultured porcine RPE cells were exposed to previously illuminated ICG and to non-illuminated ICG and kept in the dark. Cytotoxicity was quantified by trypan-blue dye-exclusion and mitochondrial dehydrogenase assay.
Results: Illumination led to a change of colour of the ICG-solution from green to yellow as a sign of molecular decomposition. There was no or mild reduction of cell viability following 60 min exposure to non-illuminated ICG (84%, controls: 100%). By contrast, 60 min exposure to illuminated ICG led to a significant reduction of cell viability to 44% (0.01% ICG), 23% (0.025% ICG) and 13% (0.05% ICG) in a dose dependent manner (n=5 each).
Conclusions: Exposure to light leads to molecular decomposition of ICG. The decomposition products are toxic to porcine RPE cells in vitro in a dose-dependent manner.

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